Part:BBa_K3762010:Design
YFP_LOV driven by T7 promoter
Design rationale
Our project design affected which part we ended up with and how we improved it. One of our initial project approaches included using a whole-cell sensor in a microfluidic chip that would detect H2S in the water that moves across the sensor. In this environment, the cell would be in near hypoxic conditions which would require a signal generator that would work without reliable access to molecular oxygen so that we could detect activity via its fluorescence signaling.
Figure 1: Plasmid map of the pENZ004 plasmid and our construct BBa_k3762010.
Design Notes
This part is compatible with most BioBrick standards.
Source
BBa_K3427000 from GO_Paris-Saclay’s 2020 team which got their protein sequence from Nazarenko et al 2019 [1]
Part order:
BBa_I746905 BBa_B0034 BBa_K3427000 BBa_B0010 BBa_B0012
References
[1] A thermostable flavin-based fluorescent protein from Chloroflexus aggregans: a framework for ultra-high resolution structural studies Nazarenko et al. 2019, Photochem
Photochem. Photobiol. Sci., 2019,18, 1793-1805